Matrices listed below were spiked with certain level of APPL2 and the recovery rates were calculated by comparing the measured value to the expected amount of APPL2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-99
96
EDTA plasma(n=5)
87-104
94
heparin plasma(n=5)
85-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APPL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
85-104%
86-96%
EDTA plasma(n=5)
90-93%
87-101%
85-92%
heparin plasma(n=5)
82-100%
86-99%
85-97%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q8NEU8
Antigen
APPL2
Applications
ELISA
Applications Notes
APPL2 ELISA Kit allows for the in vitro quantitative determination of APPL2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human APPL2(DCC-Interacting Protein 13-Beta) ELISA Kit