Human Apoptosis Protease Activating Factor-1

Mfr.No.

E1835Hu

Description

This sandwich kit is for the accurate quantitative detection of human Apoptosis Protease Activating Factor-1 (also known as Apaf-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

APAF-1;Apoptotic protease-activating factor 1;CED4

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

141840

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

8.14ng/L

Background

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Gene ID	317
UniProt ID	O14727
Antigen	Apaf-1

Research Area

Cell Biology

Protocol

These standard curves of E1835Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.393	2.278	2.233
	2.163
800ng/L	1.377	1.406	1.36
	1.434
400ng/L	0.625	0.652	0.607
	0.679
200ng/L	0.333	0.335	0.29
	0.337
100ng/L	0.135	0.17	0.125
	0.205
0ng/L	0.042	0.045	0
	0.049

Precision

We measured random samples of E1835Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	228.97	7.52	3.28
Intra-Assay	2	18	581.47	24.18	4.16
Intra-Assay	3	18	774.99	19.51	2.52

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103409	E1835Hu	48T	$320		Add to Cart
BHE12103409	E1835Hu	96T	$458		Add to Cart

Human Apoptosis Protease Activating Factor-1 - APAF-1 ELISA Kit

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