Human Apolipoprotein B100 - APO-B100 ELISA Kit

Mfr.No.

E1536Hu

Description

This sandwich kit is for the accurate quantitative detection of human Apoprotein B100 (also known as APO-B100) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Apo B-100;Apo B-48;APOB;Apolipoprotein B-100;Apolipoprotein B-48

Reactivity

Human

Detection Range

0.03-6mg/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

515605

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.01mg/ml

Background

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Gene ID	338
UniProt ID	P04114
Antigen	APOB

Research Area

Cardiovascular; Signal Transduction

Protocol

These standard curves of E1536Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3.2mg/ml	2.646	2.626	2.579
	2.607
1.6mg/ml	1.547	1.546	1.498
	1.544
0.8mg/ml	0.869	0.806	0.758
	0.742
0.4mg/ml	0.531	0.47	0.423
	0.41
0.2mg/ml	0.217	0.212	0.164
	0.207
0mg/ml	0.046	0.048	0
	0.049

Precision

We measured random samples of E1536Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.4	0.04	2.9
Intra-Assay	2	18	0.9	0.05	5.4
Intra-Assay	3	18	0.4	0.02	5.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103150	E1536Hu	48T	$320		Add to Cart
BHE12103150	E1536Hu	96T	$458		Add to Cart

Human Apolipoprotein B100 - APO-B100 ELISA Kit

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