Human apolipoprotein B100 (Apo-B100) ELISA Kit

Mfr.No.

CSB-E08100h

Alternative Names

apolipoprotein B (including Ag(x) antigen), FLDB, LDLCQ4, apoB-100, apoB-48, apolipoprotein B, apolipoprotein B48

Reactivity

Human

Detection Range

78 ng/mL-5000 ng/mL

Sample Type

Serum, Plasma, Saliva, Cell culture supernatants, Urine

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse LDH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-108
EDTA plasma (n=4)	85	81-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	100
	Range %	95-106
1:200	Average %	88
	Range %	84-93
1:400	Average %	107
	Range %	103-110
1:800	Average %	86
	Range %	83-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

19.5 ng/mL

Limit Of Detection

450 nm

UniProt ID	P04114
Antigen	apolipoprotein B (including Ag(x) antigen)

Applications

ELISA

Research Area

APOB

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501395	CSB-E08100h	96T	$495	Add to Cart
BHE10501395	CSB-E08100h	5x96T	$2376	Add to Cart
BHE10501395	CSB-E08100h	10x96T	$4702.50	Add to Cart