Matrices listed below were spiked with certain level of APOE and the recovery rates were calculated by comparing the measured value to the expected amount of APOE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
96
EDTA plasma(n=5)
85-105
96
heparin plasma(n=5)
85-96
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APOE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
APOE ELISA Kit allows for the in vitro quantitative determination of APOE concentrations in serum, plasma, tissue homogenates and other biological fluids.