Matrices listed below were spiked with certain level of APOA4 and the recovery rates were calculated by comparing the measured value to the expected amount of APOA4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
93
EDTA plasma(n=5)
85-105
96
heparin plasma(n=5)
88-97
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APOA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
APOA4 ELISA Kit allows for the in vitro quantitative determination of APOA4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human APOA4(Apolipoprotein A-IV) ELISA Kit