Matrices listed below were spiked with certain level of APLN36 and the recovery rates were calculated by comparing the measured value to the expected amount of APLN36 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
97
EDTA plasma(n=5)
89-102
95
heparin plasma(n=5)
87-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APLN36 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
APLN36 ELISA Kit allows for the in vitro quantitative determination of APLN36 concentrations in serum, plasma, tissue homogenates and other biological fluids.