Matrices listed below were spiked with certain level of APIP and the recovery rates were calculated by comparing the measured value to the expected amount of APIP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
81-105
93
EDTA plasma(n=5)
83-94
90
heparin plasma(n=5)
81-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
80-94%
92-98%
EDTA plasma(n=5)
92-99%
90-99%
94-100%
heparin plasma(n=5)
91-99%
90-100%
91-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q96GX9
Applications
ELISA
Applications Notes
APIP ELISA Kit allows for the in vitro quantitative determination of APIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human APIP(Methylthioribulose-1-phosphate dehydratase) ELISA Kit