Human APAF1(Apoptosis Protease Activating Factor 1) ELISA Kit

Mfr.No.

EH2654

Alternative Names

APAF1, APAF-1apoptotic protease activating factor 1, apoptotic peptidase activating factor, apoptotic peptidase activating factor 1, apoptotic protease activating factor, apoptotic protease-activating factor 1, CED4, DKFZp781B1145, KIAA0413 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of APAF1 and the recovery rates were calculated by comparing the measured value to the expected amount of APAF1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-104	97
EDTA plasma(n=5)	85-105	92
heparin plasma(n=5)	86-105	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APAF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-99%	87-101%	87-105%
EDTA plasma(n=5)	85-98%	85-101%	91-98%
heparin plasma(n=5)	82-98%	89-98%	84-96%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

APAF1/APAF-1apoptotic protease activating factor 1/apoptotic peptidase activating factor/apoptotic peptidase activating factor 1/apoptotic protease activating factor/apoptotic protease-activating factor 1/CED4/DKFZp781B1145/KIAA0413

Notes

For Research Use Only

UniProt ID	O14727
Antigen	APAF1

Applications	ELISA
Applications Notes	APAF1 ELISA Kit allows for the in vitro quantitative determination of APAF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10803538	EH2654	96T	$400		Add to Cart

Human APAF1(Apoptosis Protease Activating Factor 1) ELISA Kit

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