Matrices listed below were spiked with certain level of AP17 and the recovery rates were calculated by comparing the measured value to the expected amount of AP17 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-105
99
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
90-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AP17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AP17 ELISA Kit allows for the in vitro quantitative determination of AP17 concentrations in serum, plasma, tissue homogenates and other biological fluids.