Human Antithrombin III(AT III)ELISA Kit

Mfr.No.

CSB-E17902h

Sensitivity

0.82 ng/mL

Detection Range

0.82 ng/mL-600 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

serpin peptidase inhibitor, clade C (antithrombin), member 1, AT3, ATIII, MGC22579, antithrombin III, serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1, serine-cysteine proteinase inhibitor clade C member 1, serpin peptidase inhibitor, c

Recovery

The recovery of pig CLPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-105
EDTA plasma (n=4)	104	88-111

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig CLPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	99
	Range %	94-103
1:4	Average %	103
	Range %	98-107
1:8	Average %	95
	Range %	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
1000	2.297	2.312	2.305	2.196
500	1.786	1.688	1.737	1.628
250	1.133	1.202	1.168	1.059
125	0.654	0.665	0.660	0.551
62.5	0.389	0.374	0.382	0.273
31.2	0.242	0.254	0.248	0.139
15.6	0.177	0.187	0.182	0.073
0	0.105	0.113	0.109	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P01008

Applications	ELISA
Research Area	SERPINC1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501368	CSB-E17902h	96T	$790	Add to Cart
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BHE10501368	CSB-E17902h	10x96T	$7505	Add to Cart

Human Antithrombin III(AT III)ELISA Kit

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