Human Antigen KI-67 (Ki-67) ELISA Kit

Mfr.No.

CSB-E16294h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

antigen identified by monoclonal antibody Ki-67, RP11-380J17.2, KIA, proliferation-related Ki-67 antigen

Recovery

The recovery of human IMP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-105
EDTA plasma (n=4)	86	80-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IMP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
2500	2.445	2.555	2.500	2.344
1250	1.791	1.861	1.826	1.670
625	1.232	1.287	1.260	1.104
312	0.866	0.877	0.872	0.716
156	0.580	0.593	0.587	0.431
78	0.437	0.424	0.431	0.275
39	0.283	0.289	0.286	0.130
0	0.155	0.156	0.156	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P46013

Applications	ELISA
Research Area	KI-67

Storage	Store at 2-8°C. Please refer to protocol.

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