Human Anti-Thrombin 3 - AT-3 ELISA Kit

Mfr.No.

E1174Hu

Sensitivity

2.21mg/dl

Detection Range

5-1000mg/dl

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Anti-Thrombin III (also known as AT-III) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Antithrombin-III;ATIII;Serpin C1;SERPINC 1;SERPINC1

Molecular Weight

52602

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1174Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	350.7	12.19	3.5
Intra-Assay	2	18	211.8	10.83	5.1
Intra-Assay	3	18	96	5.45	5.7

Background

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.

Protocol

These standard curves of E1174Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
560mg/dl	2.172	2.282	2.212
	2.391
280mg/dl	1.38	1.415	1.346
	1.45
140mg/dl	0.706	0.802	0.733
	0.899
70mg/dl	0.406	0.461	0.392
	0.516
35mg/dl	0.234	0.239	0.17
	0.244
0mg/dl	0.071	0.069	0
	0.068

Gene ID	462
UniProt ID	P01008

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102821	E1174Hu	48T	$320		Add to Cart
BHE12102821	E1174Hu	96T	$458		Add to Cart

Human Anti-Thrombin 3 - AT-3 ELISA Kit

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