Human Anti-Thrombin 3 - AT-3 ELISA Kit

Mfr.No.

E1174Hu

Description

This sandwich kit is for the accurate quantitative detection of human Anti-Thrombin III (also known as AT-III) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Antithrombin-III;ATIII;Serpin C1;SERPINC 1;SERPINC1

Reactivity

Human

Detection Range

5-1000mg/dl

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

52602

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.21mg/dl

Background

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.

Gene ID	462
UniProt ID	P01008
Antigen	SERPINC1

Research Area

Cardiovascular

Protocol

These standard curves of E1174Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
560mg/dl	2.172	2.282	2.212
	2.391
280mg/dl	1.38	1.415	1.346
	1.45
140mg/dl	0.706	0.802	0.733
	0.899
70mg/dl	0.406	0.461	0.392
	0.516
35mg/dl	0.234	0.239	0.17
	0.244
0mg/dl	0.071	0.069	0
	0.068

Precision

We measured random samples of E1174Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	350.7	12.19	3.5
Intra-Assay	2	18	211.8	10.83	5.1
Intra-Assay	3	18	96	5.45	5.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102821	E1174Hu	48T	$320		Add to Cart
BHE12102821	E1174Hu	96T	$458		Add to Cart

Human Anti-Thrombin 3 - AT-3 ELISA Kit

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