Matrices listed below were spiked with certain level of SARS-CoV2(S1 protein) IgG and the recovery rates were calculated by comparing the measured value to the expected amount of SARS-CoV2(S1 protein) IgG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
91
EDTA plasma(n=5)
80-92
85
heparin plasma(n=5)
83-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SARS-CoV2(S1 protein) IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
84-105%
81-99%
91-105%
EDTA plasma(n=5)
81-100%
89-100%
80-94%
heparin plasma(n=5)
81-99%
81-91%
80-98%
Sensitivity
< 4.688ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-SARS-CoV2 IgG ELISA Kit allows for the in vitro quantitative determination of SARS-CoV2(S1 protein) IgG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti-SARS-CoV2(S1 protein) IgG ELISA Kit