Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) and the recovery rates were calculated by comparing the measured value to the expected amount of anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
97
EDTA plasma(n=5)
89-99
95
heparin plasma(n=5)
86-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-98%
85-100%
85-100%
EDTA plasma(n=5)
88-100%
83-100%
84-99%
heparin plasma(n=5)
81-94%
83-100%
88-100%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-SARS-CoV2 IgM ELISA Kit allows for the in vitro quantitative determination of anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti-SARS-CoV2 (S-RBD) (Omicron,B.1.1.529) IgM ELISA Kit