This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombined nCoV Nucleocapsid protein (antigen) was pre-coated onto 96-well plates. The test samples were added to the wells, unbound conjugates were washed away with wash buffer. Then added HRP conjugated anti-human igM, if there were any nCoV(N) -igM antibody in the samples, it would form a complex. TMB substrates were used to visualize HRP enzymatic reaction. It was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
Notes
For Research Use Only
Antigen
2019 nCoV(N) IgM
Applications
ELISA
Applications Notes
anti-SARS-CoV2(N) IgM ELISA Kit allows for the in vitro quantitative determination of anti-SARS-CoV2 IgM concentrations in plasma, tissue homogenates and other biological fluids.