Human Anti-nuclear Antibody,ANA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09075h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

Anti-nuclear Antibody,ANA

Recovery

The recovery of sheep GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	94	89-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-102
1:4	Average %	100
	Range %	94-106
1:8	Average %	95
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ANA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	ESPS Manuscript NO: 30283 Manuscript Type: ORIGINAL ARTICLE Basic Study Autoantibody profiles in autoimmune hepatitis and chronic hepatitis C Kawa Amin.et al,World Journal of Gastroenterology,Dec 8 Autoantibody profiles in autoimmune hepatitis and chronic hepatitis C identifies similarities in patients with severe disease Kawa Amin.et al,World J Gastroenterol.,Feb 28.

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BHE10501318	CSB-E09075h	96T	$495	Add to Cart
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BHE10501318	CSB-E09075h	10x96T	$4702.50	Add to Cart