Human anti-mutated citrullinated vimentin (MCV) antibody ELISA Kit

Mfr.No.

CSB-E09565h

Alternative Names

anti-mutated citrullinated vimentin (MCV) antibody

Reactivity

Human

Detection Range

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Sample Type

Serum, Plasma

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	98
	Range %	94-103
1:200	Average %	86
	Range %	83-90
1:400	Average %	104
	Range %	100-108
1:800	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average
2000	0.111	0.115	0.113
1000	0.208	0.210	0.209
500	0.373	0.390	0.382
250	0.642	0.638	0.640
125	0.956	0.961	0.959
62.5	1.146	1.200	1.173
31.25	1.417	1.431	1.424
0	1.634	1.645	1.640

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

Antigen	anti-mutated citrullinated vimentin (MCV) antibody

Applications

ELISA

Research Area

MCV Ab

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Diagnostic Usefulness of Anti-Cyclic Citrullinated Peptide and Anti-Mutated Citrullinated Vimentin Antibodies in the Diagnosis of Seronegative Rheumatoid Arthritis Patients Bushra Gohar Shah.et al,Journal of Islamic International Medical College,11(1): 3-7 Type-III interferons and rheumatoid arthritis: Correlation between interferon lambda 1 (interleukin 29) and antimutated citrullinated vimentin antibody levels Castillo-Martnez, Diana.et al,autoimmunity ,Feb 14

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SKU	Supplier No.	Size	Your price
BHE10501310	CSB-E09565h	96T	$695	Add to Cart
BHE10501310	CSB-E09565h	5x96T	$3336	Add to Cart
BHE10501310	CSB-E09565h	10x96T	$6602.50	Add to Cart

Human anti-mutated citrullinated vimentin (MCV) antibody ELISA Kit

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