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Home / ELISA Kit

Human Anti-Mullerian hormone (AMH) ELISA Kit

SKU : BHE13704843

Anti-Müllerian hormone (AMH) is a dimeric glycoprotein that inhibits the development of the Müllerian ducts in a male embryo. It is named after Johannes Peter Müller. AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-beta (TGF-beta) family. It is present in fish, reptiles, birds, marsupials, and placental mammals.In mammals AMH is secreted by Sertoli cells of the testes during embryogenesis of the fetal male and prevents the development of the mullerian ducts into the uterus and other mullerian structures. The effect is ipsilateral, that is each testis suppresses Müllerian development only on its own side. In humans this action takes place by 8 weeks gestation. In female embryogenesis the absence of AMH allows for the development of upper vagina, uterus and cervix, and oviducts.

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Mfr.No.	KTE62640
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Anti-Mullerian hormone (AMH) ELISA Kit employs a two-site sandwich ELISA to quantitate AMH.
Alternative Names	AMH; MIF; MIS; Mullerian inhibiting factor; Mullerian inhibiting substance
Detection Method	Colorimetric
Kit Components	Human Anti-Mullerian hormone microplate Human Anti-Mullerian hormone standard Human Anti-Mullerian hormone detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Anti-Mullerian hormone (AMH) ELISA Kit has high sensitivity and excellent specificity for detection of Human AMH. No significant cross-reactivity or interference between Human AMH and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Anti-Müllerian hormone (AMH) is a dimeric glycoprotein that inhibits the development of the Müllerian ducts in a male embryo. It is named after Johannes Peter Müller. AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-beta (TGF-beta) family. It is present in fish, reptiles, birds, marsupials, and placental mammals.In mammals AMH is secreted by Sertoli cells of the testes during embryogenesis of the fetal male and prevents the development of the mullerian ducts into the uterus and other mullerian structures. The effect is ipsilateral, that is each testis suppresses Müllerian development only on its own side. In humans this action takes place by 8 weeks gestation. In female embryogenesis the absence of AMH allows for the development of upper vagina, uterus and cervix, and oviducts.
Notes	This Human Anti-Mullerian hormone (AMH) ELISA Kit employs a two-site sandwich ELISA to quantitate AMH in samples. An antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	280718

Applications	ELISA
Applications Notes	This Human Anti-Mullerian hormone (AMH) ELISA Kit employs a two-site sandwich ELISA to quantitate AMH in samples. An antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704843	KTE62640	48 T	$359		Add to Cart
BHE13704843	KTE62640	96 T	$619		Add to Cart
BHE13704843	KTE62640	96 Tx5	$2809		Add to Cart
BHE13704843	KTE62640	96 Tx50	$26209		Add to Cart

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