Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of anti-MMP-2 and the recovery rates were calculated by comparing the measured value to the expected amount of anti-MMP-2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
99
EDTA plasma(n=5)
88-105
98
heparin plasma(n=5)
91-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-MMP-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-103%
85-99%
87-105%
EDTA plasma(n=5)
86-93%
83-101%
84-99%
heparin plasma(n=5)
82-99%
86-100%
82-98%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-MMP-2 ELISA Kit allows for the in vitro quantitative determination of anti-MMP-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.