Matrices listed below were spiked with certain level of anti-MBP and the recovery rates were calculated by comparing the measured value to the expected amount of anti-MBP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
97
EDTA plasma(n=5)
95-105
99
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-MBP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-104%
86-105%
86-98%
EDTA plasma(n=5)
88-99%
82-89%
87-95%
heparin plasma(n=5)
92-99%
88-94%
83-89%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-MBPELISA Kit allows for the in vitro quantitative determination of anti-MBP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti-MBP(anti-myelin basic protein antibody)ELISA Kit