Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

Mfr.No.

ELK0814

Sensitivity

1.71 U/mL

Detection Range

4.69-300 U/mL

Reactivity

Human

Sample Type

Serum, Plasma, Other biological fluids

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

ANTI-LKM1

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-LKM1 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-LKM1 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-98%	90%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	89-103%	96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-LKM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-93%	91-105%	87-98%	87-103%
EDTA plasma(n=5)	86-92%	89-103%	89-98%	79-85%
Heparin plasma(n=5)	93-99%	86-101%	87-96%	92-105%

Typical Data

Concentration (U/mL)	OD	Corrected OD
300.00	2.096	2.008
150.00	1.638	1.550
75.00	1.182	1.094
37.50	0.871	0.783
18.75	0.539	0.451
9.38	0.326	0.238
4.69	0.226	0.138
0.00	0.088	0.000

Limit Of Detection

300 U/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-LKM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-LKM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-LKM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-LKM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208459	ELK0814	48T	$320		Add to Cart
BHE15208459	ELK0814	96T	$458		Add to Cart

Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

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