Matrices listed below were spiked with certain level of Anti LH and the recovery rates were calculated by comparing the measured value to the expected amount of Anti LH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
96
EDTA plasma(n=5)
94-105
98
heparin plasma(n=5)
88-99
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti LH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-101%
88-104%
87-101%
EDTA plasma(n=5)
86-101%
84-99%
89-100%
heparin plasma(n=5)
82-93%
80-91%
84-99%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Anti LH ELISA Kit allows for the in vitro quantitative determination of Anti LH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Anti LH (ANTI-Luteinizing Hormone antibody)ELISA Kit