Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of anti-LAMA5 antibody and the recovery rates were calculated by comparing the measured value to the expected amount of anti-LAMA5 antibody in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
96
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
85-96
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-LAMA5 antibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-105%
91-101%
89-105%
EDTA plasma(n=5)
84-90%
83-91%
84-100%
heparin plasma(n=5)
86-99%
88-100%
83-96%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-LAMA5antibody Elisa kit allows for the in vitro quantitative determination of anti-LAMA5 antibody concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti-LAMA5 (Laminin subunit alpha-5) antibody Elisa kit