Matrices listed below were spiked with certain level of Anti-KLHL12 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-KLHL12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
93
EDTA plasma(n=5)
87-104
95
heparin plasma(n=5)
87-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-KLHL12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-103%
93-103%
94-104%
EDTA plasma(n=5)
83-100%
83-98%
83-101%
heparin plasma(n=5)
80-99%
81-99%
87-98%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
UniProt ID
Q53G59
Applications
ELISA
Applications Notes
Anti-KLHL12ELISA Kit allows for the in vitro quantitative determination of Anti-KLHL12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Anti-KLHL12(anti-Kelch-like protein 12 antibody)ELISA Kit