Matrices listed below were spiked with certain level of anti-Histone and the recovery rates were calculated by comparing the measured value to the expected amount of anti-Histone in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-99
93
EDTA plasma(n=5)
93-100
97
heparin plasma(n=5)
86-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-Histone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-105%
87-104%
85-101%
EDTA plasma(n=5)
85-100%
85-97%
86-101%
heparin plasma(n=5)
85-93%
85-97%
86-100%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-Histoneantibody ELISA kit allows for the in vitro quantitative determination of anti-Histone concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti-Histone(Histone)antibody ELISA kit