Matrices listed below were spiked with certain level of anti ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of anti ET-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
95
EDTA plasma(n=5)
89-105
96
heparin plasma(n=5)
85-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
91-105%
85-99%
EDTA plasma(n=5)
90-100%
83-98%
83-98%
heparin plasma(n=5)
80-94%
81-99%
84-98%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti ET-1ELISA Kit allows for the in vitro quantitative determination of anti ET-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti ET-1(anti-Endothelin 1 IgG Antibody)ELISA Kit