Human anti-endothelial cell antibody,AECA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08691h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

anti-endothelial cell antibody,AECA

Recovery

The recovery of human GPX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	95	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GPX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	91
	Range %	88-94
1:400	Average %	105
	Range %	100-107
1:800	Average %	94
	Range %	89-97
1:1600	Average %	98
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	AECA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Endothelial antibody levels in the sera of children with autism spectrum disorders Bashir S. et al,J Chin Med Assoc,2015 Jul;78(7):414-7. Differences in immune responses between CMV-seronegative and-seropositive patients with myocardial ischemia and reperfusion Evgeniya V. Shmeleva et al,Immunity, Inflammation and Disease,2015 Jun;3(2):56-70 Correlation of retinol binding protein 4 with metabolic indexes of glucose and lipid, bile cholesterol saturation index WANG Wen et al,Journal of Central South University(Medical Science),2015 (6)

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