Matrices listed below were spiked with certain level of anti-CEA and the recovery rates were calculated by comparing the measured value to the expected amount of anti-CEA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
95
EDTA plasma(n=5)
85-102
92
heparin plasma(n=5)
88-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-CEA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
96-104%
85-97%
87-104%
EDTA plasma(n=5)
85-101%
88-101%
85-94%
heparin plasma(n=5)
85-96%
86-97%
87-100%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-CEA antibody Elisa kit allows for the in vitro quantitative determination of anti-CEA concentrations in serum, plasma, tissue homogenates and other biological fluids.