Human anti-Bullous Pemphigoid 180 antibody(anti-BP180-Ab) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09505h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Alternative Names

anti-Bullous Pemphigoid 180 antibody(anti-BP180-Ab)

Recovery

The recovery of human ALT/GPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ALT/GPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	84
	Range %	80-88
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	anti-BP180-Ab

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10501230	CSB-E09505h	96T	$695	Add to Cart
BHE10501230	CSB-E09505h	5x96T	$3336	Add to Cart
BHE10501230	CSB-E09505h	10x96T	$6602.50	Add to Cart