Human anti-beta1-adrenergic receptor(beta1AR) autoantibody ELISA Kit

Mfr.No.

CSB-E15079h

Alternative Names

anti-beta1-adrenergic receptor(beta1AR) autoantibody, anti-beta1-adrenergic receptor(beta1AR) autoantibody, beta1AR

Reactivity

Human

Detection Range

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Sample Type

Serum

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of mouse GRIN2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	87	83-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRIN2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-96
1:2	Average %	96
	Range %	92-100
1:4	Average %	105
	Range %	100-114
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.683	2.579	2.631	2.485
1000	2.159	2.052	2.106	1.960
500	1.494	1.521	1.508	1.362
250	0.916	0.869	0.893	0.747
125	0.587	0.607	0.597	0.451
62.5	0.373	0.383	0.378	0.232
31.25	0.265	0.255	0.260	0.114
0	0.149	0.142	0.146	?

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

Antigen	anti-β1-adrenergic receptor(β1AR) autoantibody

Applications

ELISA

Research Area

beta1AR Ab

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Detection of DCM-associated B1-adrenergic receptor autoantibodies requires functional readouts or native human B1-receptors as targets Bornholz B.et al,Int J Cardiol.,2015 202:728-730. Exploring the relationship between B-adrenergic receptor kinase-1 and physical symptoms in heart failure Quin E.et al,Heart & Lung,May 24

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SKU	Supplier No.	Size	Your price
BHE10501381	CSB-E15079h	96T	$695	Add to Cart
BHE10501381	CSB-E15079h	5x96T	$3336	Add to Cart
BHE10501381	CSB-E15079h	10x96T	$6602.50	Add to Cart

Human anti-beta1-adrenergic receptor(beta1AR) autoantibody ELISA Kit

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