Matrices listed below were spiked with certain level of anti-ASIC1 and the recovery rates were calculated by comparing the measured value to the expected amount of anti-ASIC1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
93
EDTA plasma(n=5)
92-105
97
heparin plasma(n=5)
91-98
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-ASIC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-104%
86-104%
85-97%
EDTA plasma(n=5)
86-98%
87-93%
97-101%
heparin plasma(n=5)
91-94%
88-97%
88-97%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
UniProt ID
P78348
Applications
ELISA
Applications Notes
anti-ASIC1antibody Elisa kit allows for the in vitro quantitative determination of anti-ASIC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human anti-ASIC1(Acid-sensing ion channel 1)antibody Elisa kit