Human Anterior gradient protein 3 homolog(AGR3) ELISA kit

Human Anterior gradient protein 3 homolog(AGR3) ELISA kit

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Mfr.No.

CSB-EL001459HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

anterior gradient homolog 3 (Xenopus laevis), AG3, BCMP11, HAG3, PDIA18, hAG-3, anterior gradient protein 3, breast cancer membrane protein 11, protein disulfide isomerase family A, member 18

Recovery

The recovery of human PGF2? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	88	81-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PGF2? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	102
	Range %	97-107
1:4	Average %	93
	Range %	85-99
1:8	Average %	98
	Range %	91-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.822	2.963	2.893	2.778
50	2.212	2.366	2.289	2.174
25	1.254	1.356	1.305	1.190
12.5	0.640	0.655	0.648	0.533
6.25	0.411	0.445	0.428	0.313
3.12	0.242	0.255	0.249	0.134
1.56	0.175	0.172	0.174	0.059
0	0.116	0.113	0.115	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8TD06

Applications	ELISA
Research Area	AGR3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501213	CSB-EL001459HU	96T	$695	Add to Cart
BHE10501213	CSB-EL001459HU	5x96T	$3336	Add to Cart
BHE10501213	CSB-EL001459HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.