Human Anosmin-1(KAL1) ELISA kit

Mfr.No.

CSB-EL011978HU

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Kallmann syndrome 1 sequence, ADMLX, HHA, KAL, KALIG-1, KMS, Kallmann syndrome 1 protein, Kallmann syndrome interval gene 1, Kallmann syndrome-1 sequence (anosmin-1), adhesion molecule-like X-linked, anosmin-1

Recovery

The recovery of human LAMC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-95
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LAMC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-110
1:2	Average %	93
	Range %	89-97
1:4	Average %	88
	Range %	84-92
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.012	2.148	2.080	1.888
25	1.701	1.793	1.747	1.555
12.5	1.412	1.332	1.372	1.180
6.25	0.901	0.945	0.923	0.731
3.12	0.633	0.601	0.617	0.425
1.56	0.412	0.438	0.425	0.233
0.78	0.305	0.314	0.310	0.118
0	0.194	0.189	0.192	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P23352

Applications	ELISA
Research Area	KAL1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501211	CSB-EL011978HU	96T	$695	Add to Cart
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BHE10501211	CSB-EL011978HU	10x96T	$6602.50	Add to Cart

Human Anosmin-1(KAL1) ELISA kit

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