Matrices listed below were spiked with certain level of ANNA1 and the recovery rates were calculated by comparing the measured value to the expected amount of ANNA1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-105
99
EDTA plasma(n=5)
86-99
93
heparin plasma(n=5)
86-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANNA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ANNA1 ELISA Kit allows for the in vitro quantitative determination of ANNA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ANNA1(Anti-Neuronal Nuclear Antibody 1) ELISA Kit