Human Ankyrin Repeat Domain-containing Protein 1

Mfr.No.

E4821Hu

Sensitivity

1.34ng/L

Detection Range

2-600ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Ankyrin Repeat Domain-containing Protein 1 (also known as ANKRD1/CARP) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ALRP;ANKRD1;Ankyrin repeat domain-containing protein 1;bA320F15.2;C-193;Cardiac ankyrin repeat protein;CARP;CVARP;Cytokine-inducible gene C-193 protein;Cytokine-inducible nuclear protein;MCARP

Molecular Weight

36252

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4821Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	208.46	6.8	3.26
Intra-Assay	2	18	66.33	3.1	4.68
Intra-Assay	3	18	75.07	3.61	4.81

Background

May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.

Protocol

These standard curves of E4821Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.352	2.346	2.294
	2.34
160ng/L	1.334	1.428	1.376
	1.521
80ng/L	0.84	0.854	0.803
	0.869
40ng/L	0.403	0.406	0.355
	0.41
20ng/L	0.31	0.269	0.218
	0.228
0ng/L	0.044	0.052	0
	0.06

Gene ID	27063
UniProt ID	Q15327

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106096	E4821Hu	48T	$320		Add to Cart
BHE12106096	E4821Hu	96T	$458		Add to Cart

Human Ankyrin Repeat Domain-containing Protein 1 - ANKRD1 ELISA Kit

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