Human Ankyrin Repeat and Socs Box Protein 2

Mfr.No.

E4952Hu

Sensitivity

3.04ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Ankyrin repeat and SOCS box protein 2;ASB 2;ASB2;ASB-2

Molecular Weight

65084

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4952Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	384.3	9.23	2.4
Intra-Assay	2	18	242.9	10.57	4.4
Intra-Assay	3	18	106.8	5.27	4.9

Background

Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. (Isoform 1): Enhances adhesion of hematopoietic cells to fibronectin through targeting of filamins FLNA and FLNB for proteasomal degradation, it is transiantly expressed during hematopoietic cell differentiation. (Isoform 2): Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA.

Protocol

These standard curves of E4952Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.469	2.49	2.437
	2.511
320ng/L	1.388	1.412	1.359
	1.437
160ng/L	0.755	0.784	0.731
	0.814
80ng/L	0.464	0.448	0.395
	0.433
40ng/L	0.234	0.256	0.204
	0.279
0ng/L	0.043	0.053	0
	0.063

Gene ID	51676
UniProt ID	Q96Q27

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106225	E4952Hu	48T	$320		Add to Cart
BHE12106225	E4952Hu	96T	$458		Add to Cart

Human Ankyrin Repeat and Socs Box Protein 2 - ASB2 ELISA Kit

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