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Home / ELISA Kit

Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit

SKU : BHE13703185

Angiotensin II receptor, type 1 or AT1 receptor is an angiotensin receptor. It has vasopressor effects and regulates aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system.It is the best elucidated angiotensin receptor.
The angiotensin receptor is activated by the vasoconstricting peptide angiotensin II. The activated receptor in turn couples to Gq/11 and thus activates phospholipase C and increases the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C. Activated receptor also inhibits adenylate cyclase and activates various tyrosine kinases.

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Mfr.No.	KTE60969
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGTR1.
Alternative Names	AGTR1; AG2S; AGTR1A; AGTR1B; AT1; AT1B; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; angiotensin receptor 1; angiotensin receptor 1B; type-1B angiotensin receptor
Detection Method	Colorimetric
Kit Components	Human Angiotension II receptor 1 microplate Human Angiotension II receptor 1 standard Human Angiotension II receptor 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGTR1. No significant cross-reactivity or interference between Human AGTR1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Angiotensin II receptor, type 1 or AT1 receptor is an angiotensin receptor. It has vasopressor effects and regulates aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system.It is the best elucidated angiotensin receptor. The angiotensin receptor is activated by the vasoconstricting peptide angiotensin II. The activated receptor in turn couples to Gq/11 and thus activates phospholipase C and increases the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C. Activated receptor also inhibits adenylate cyclase and activates various tyrosine kinases.
Notes	This Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGTR1 in samples. An antibody specific for AGTR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGTR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGTR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGTR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	185

Applications	ELISA
Applications Notes	This Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGTR1 in samples. An antibody specific for AGTR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGTR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGTR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGTR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703185	KTE60969	48 T	$339		Add to Cart
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