Human Angiotensin II Receptor 2 antibody,AT2R-Ab ELISA Kit

Mfr.No.

CSB-E11255h

Sensitivity

0.078 U/mL

Detection Range

0.312 U/mL-20 U/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Angiotensin II Receptor 2 antibody,AT2R-Ab, angiotensin 2 receptor 2 antibody, AT2R-Ab

Recovery

The recovery of human HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-102
EDTA plasma (n=4)	91	85-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

84-99

1:2

Average %

Range %

82-94

1:4

Average %

Range %

89-102

1:8

Average %

Range %

88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	1.912	1.831	1.872	1.780
2500	1.454	1.369	1.412	1.320
1250	0.839	0.789	0.814	0.722
625	0.456	0.487	0.472	0.380
312	0.283	0.297	0.290	0.198
156	0.199	0.202	0.201	0.109
78	0.103	0.108	0.106	0.014
0	0.089	0.094	0.092	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	AT2R Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501194	CSB-E11255h	96T	$695	Add to Cart
BHE10501194	CSB-E11255h	5x96T	$3336	Add to Cart
BHE10501194	CSB-E11255h	10x96T	$6602.50	Add to Cart

Human Angiotensin II Receptor 2 antibody,AT2R-Ab ELISA Kit

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