Human angiotensin II receptor 1 Antibody,ATIIR1 Ab ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10374h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

angiotensin II receptor 1 Antibody,ATIIR1 Ab, angiotensin 2 receptor 1 antibody, AT2R1-Ab

Recovery

The recovery of mouse HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	95	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	80-98
1:200	Average %	85
	Range %	81-96
1:400	Average %	97
	Range %	90-104
1:800	Average %	95
	Range %	82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ATIIR1 Ab

Storage	Store at 2-8°C. Please refer to protocol.

Reference	De novo development of antibodies to kidney-associated self-antigens angiotensin II receptor type I, collagen IV, and fibronectin occurs at early time points after kidney transplantation in children. /,Pediatr Transplant.,2015 Aug;19(5):499-503.

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