Human angiotensin II (ANG-II) ELISA Kit

Human angiotensin II (ANG-II) ELISA Kit

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Mfr.No.

CSB-E04493h

Alternative Names

angiotensin II, angiotensin II, angiotensin 2, ANG2

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	92
	Range %	87-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.536	2.598	2.567	2.405
10	1.735	1.768	1.752	1.590
5	1.036	1.098	1.067	0.905
2.5	0.650	0.698	0.674	0.512
1.25	0.446	0.432	0.439	0.277
0.625	0.342	0.358	0.350	0.188
0.312	0.240	0.261	0.251	0.089
0	0.161	0.163	0.162	?

Species

Homo sapiens

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

Antigen	Angiotensin II

Applications

ELISA

Research Area

ANG-II

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Therapeutic effect of forest bathing on human hypertension in the elderly Mao GX et al,J Cardiol,2012 Dec;60(6):495-502. Differential regulation of mitogen-activated protein kinase signaling pathways in human with different types of mitral valvular disease Chang C et al ,Journal of Surgical Research,2013 May 1;181(1):49-59 Evaluation of Serum Levels of Renin Angiotensin System Components in Asthmatic Patients Ceylan Ayada1. et al,Erciyes Med J ,37(3): 87-90 OR21 Is circulating angiotensin II receptor a potentialMarker of renal allograft injury? Pam Kimball. et al,Human Immunology,2015 October; 76,Pages 19 Propofol prevents human umbilical vein endothelial cell injury from Ang II-induced apoptosis by activating the ACE2-(1-7)-Mas axis and eNOS phosphorylation Liangqing Zhang .et al,Plos one,July 11

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SKU	Supplier No.	Size	Your price
BHE10501191	CSB-E04493h	96T	$725	Add to Cart
BHE10501191	CSB-E04493h	5x96T	$3480	Add to Cart
BHE10501191	CSB-E04493h	10x96T	$6887.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.