Human Angiotensin 1-7(Ang1-7)ELISA Kit

Mfr.No.

CSB-E14242h

Alternative Names

Angiotensin 1-7(Ang1-7

Reactivity

Human

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PI16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-105
EDTA plasma (n=4)	95	86-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PI16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	83-98
1:2	Average %	95
	Range %	88-105
1:4	Average %	90
	Range %	84-105
1:8	Average %	95
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	?
10	2.254	2.266	2.260	?
5	1.342	1.354	1.348	?
2.5	0.662	0.669	0.666	?
0.625	0.197	0.211	0.204	?
0.312	0.099	0.102	0.101	?

Species

Homo sapiens

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

Antigen	Angiotensin 1-7（Ang1-7

Applications

ELISA

Research Area

Ang1-7

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<20%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Evaluation of Serum Levels of Renin Angiotensin System Components in Asthmatic Patients Ceylan Ayada1. et al,Erciyes Med J ,37(3): 87-90 Angiogenic factors are associated with development of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation Nie DM. et al,J Huazhong Univ Sci Technolog Med Sci,2015 35(5):694-699

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SKU	Supplier No.	Size	Your price
BHE10501188	CSB-E14242h	96T	$695	Add to Cart
BHE10501188	CSB-E14242h	5x96T	$3336	Add to Cart
BHE10501188	CSB-E14242h	10x96T	$6602.50	Add to Cart

Human Angiotensin 1-7(Ang1-7)ELISA Kit

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