Human Angiopoietin-like Protein 8

Mfr.No.

E3372Hu

Description

This sandwich kit is for the accurate quantitative detection of human Angiopoietin-like Protein 8 (also known as ANGPTL8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Angiopoietin-like protein 8;ANGPTL 8;ANGPTL8;Betatrophin;Lipasin;Refeeding-induced fat and liver protein

Reactivity

Human

Detection Range

5-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

22105

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.36ng/L

Background

Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels (PubMed: 22569073, PubMed: 22809513, PubMed: 23150577). May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state (By similarity).

Gene ID	55908
UniProt ID	Q6UXH0
Antigen	ANGPTL8

Research Area

Others

Protocol

These standard curves of E3372Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.231	2.138	2.1
	2.046
600ng/L	1.344	1.301	1.263
	1.258
300ng/L	0.76	0.714	0.676
	0.668
150ng/L	0.525	0.527	0.489
	0.529
75ng/L	0.199	0.191	0.153
	0.183
0ng/L	0.036	0.038	0
	0.041

Precision

We measured random samples of E3372Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	856.3	25.93	3
Intra-Assay	2	18	253	15.43	6.1
Intra-Assay	3	18	490	25.41	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104901	E3372Hu	48T	$320		Add to Cart
BHE12104901	E3372Hu	96T	$458		Add to Cart

Human Angiopoietin-like Protein 8 - ANGPTL8 ELISA Kit

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