Human angiopoietin-like protein 8(ANGPTL8) ELISA Kit

Human angiopoietin-like protein 8(ANGPTL8) ELISA Kit

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Mfr.No.

CSB-EL028107HU

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

angiopoietin-like protein 8(ANGPTL8), Betatrophin,Lipasin,C19orf80,UNQ599,PRO1185

Recovery

The recovery of human GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-98
EDTA plasma (n=4)	94	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	88
	Range %	81-93
1:4	Average %	90
	Range %	85-96
1:8	Average %	97
	Range %	83-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.699	2.752	2.726	2.579
900	1.879	1.906	1.893	1.746
450	1.121	1.200	1.161	1.014
225	0.548	0.545	0.547	0.400
112.5	0.306	0.332	0.319	0.172
56	0.225	0.238	0.232	0.085
28	0.213	0.204	0.209	0.062
0	0.151	0.142	0.147	?
		?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q6UXH0
Tissue Specificity	Predominantly expressed in liver. Also expressed in adipose tissues.

Applications	ELISA
Research Area	ANGPTL8

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Circulating betatrophin levels are increased in anorexia andDecreased in morbid obese women Barja-Feaacutendez S. et al,J Clin Endocrinol Metab,2015 Jul 14:JC2015 1595. Circulating Betatrophin Levels Are Increased in Anorexia andDecreased in Morbidly Obese Women Barja-Fendez S.et al,J Clin Endocrinol Metab.,100(9):E1188-96 Circulating Betatrophin in Patients with Type 2 Diabetes: A Meta-Analysis S Li. et al,J Diabetes Res,2015 June 15 Therapeutic effects of C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO-Me; bardoxolone methyl) on radiation-induced lung inflammation and fibrosis in mice Wang YY. et al,Drug Des Devel Ther,2015 Jun 22;9:3163-78. Elevated circulating levels of betatrophin are associated with polycystic ovary syndrome. Calan M. et al,Endocrine.,Feb 1. Circulating ANGPTL8/Betatrophin Concentrations Are Increased After Surgically Induced Weight Loss, but Not After Diet-Induced Weight Loss Pascual-Corrales E. et al,Obes Surg,2016 Jan 14. Circulating betatrophin concentration is negatively correlated with insulin resistance in obese children and adolescents. Tuhan H.et al,Diabetes Res Clin Pract.,114:37-42. The Effects of Serum ANGPTL8/betatrophin on the Risk of Developing the Metabolic Syndrome - A Prospective Study. Wang H.et al,Sci Rep.,6:28431 Characterisation of betatrophin concentrations in childhood and adolescent obesity and insulin resistance Wu S.et al,Pediatr Diabetes.,2016 17(1):53-60. ANGPTL8 (Betatrophin) Role in Diabetes and Metabolic Diseases Abu-Farha M.et al,Diabetes Metab Res Rev,Jul 19 ANGPTL8 as a new determinant of type 2 diabetes remission after bariatric surgery Miriam Ejarquea.et al,Translational Research,Mar 9 Assessment of circulating betatrophin levels in intrahepatic cholestasis of pregnancy Kara.et al,J MATERN-FETAL NEO ,June 11

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.