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Home / ELISA Kit

Human angiopoietin-like protein 2(ANGPTL2) ELISA kit

SKU : BHE10501179

This Human PI3K ELISA Kit was designed for the quantitative measurement of Human PI3K protein in serum, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E13881h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

angiopoietin-like 2, RP11-1M19.1, ARP2, HARP, MGC8889, angiopoietin-related protein 2

Recovery

The recovery of human PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		98		92-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		88
		Range %		84-98
1:2		Average %		98
		Range %		91-105
1:4		Average %		92
		Range %		85-99
1:8		Average %		86
		Range %		80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.942	2.900	2.921	2.827
20	2.289	2.278	2.284	2.190
10	1.576	1.545	1.561	1.467
5	0.788	0.768	0.778	0.684
2.5	0.472	0.445	0.459	0.365
1.25	0.293	0.278	0.286	0.192
0.625	0.218	0.205	0.212	0.118
0	0.096	0.092	0.094	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	Q9UKU9

Applications	ELISA
Research Area	ANGPTL2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501179	CSB-E13881h	96T	$695		Add to Cart
BHE10501179	CSB-E13881h	5x96T	$3336		Add to Cart
BHE10501179	CSB-E13881h	10x96T	$6602.50		Add to Cart

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