Human Angiopoietin-2 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0657

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.095	0.178	0.21	0.376	0.519	0.97	1.588	2.01

Description

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANGPT2 in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva and human milk.

Alternative Names

Angiopoietin-2; ANG-2; ANGPT2;

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-F496

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Angiopoietin-2

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	327	1634	5900	341	1769	5438
Standard deviation	17.33	114.38	354	23.52	127.36	342.59
CV (%)	5.3%	7%	6%	6.9%	7.2%	6.3%

Protein Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Background

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	285
Gene Name	ANGPT2
UniProt ID	O15123

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Growth Factors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000076	EZ0657	5 plates/kit	$500		Add to Cart

Human Angiopoietin-2 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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