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Home / ELISA Kit

Human Angiogenic factor with G patch and FHA domains 1 (AGGF1) ELISA Kit

SKU : BHE13703178

The AGGF1 gene encodes a potent angiogenic factor that contains a forkhead-associated domain and a G-patch domain. VG5Q was ubiquitously expressed in human tissues examined, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Intracellular localization studies showed VG5Q protein mostly in the cytoplasm and around the nuclei of HMVECs. In an in vitro model of angiogenesis where endothelial cells were plated onto matrigel, VG5Q protein began to redistribute by moving towards the cell periphery and was also detected outside the cell. Purified wildtype VG5Q protein promoted strong angiogenesis in a chick chorioallantoic membrane assay, demonstrating that VG5Q is a potent angiogenic factor. Angiogenesis was inhibited by RNA interference against VG5Q in in vitro assays.

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Mfr.No.	KTE60962
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Angiogenic factor with G patch and FHA domains 1 (AGGF1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGGF1.
Alternative Names	AGGF1; FLJ10283; GPATC7; GPATCH7; HSU84971; HUS84971; VG5Q; angiogenic factor VG5Q; vasculogenesis gene on 5q
Detection Method	Colorimetric
Kit Components	Human Angiogenic factor with G patch and FHA domains 1 microplate Human Angiogenic factor with G patch and FHA domains 1 standard Human Angiogenic factor with G patch and FHA domains 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Angiogenic factor with G patch and FHA domains 1 (AGGF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGGF1. No significant cross-reactivity or interference between Human AGGF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The AGGF1 gene encodes a potent angiogenic factor that contains a forkhead-associated domain and a G-patch domain. VG5Q was ubiquitously expressed in human tissues examined, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Intracellular localization studies showed VG5Q protein mostly in the cytoplasm and around the nuclei of HMVECs. In an in vitro model of angiogenesis where endothelial cells were plated onto matrigel, VG5Q protein began to redistribute by moving towards the cell periphery and was also detected outside the cell. Purified wildtype VG5Q protein promoted strong angiogenesis in a chick chorioallantoic membrane assay, demonstrating that VG5Q is a potent angiogenic factor. Angiogenesis was inhibited by RNA interference against VG5Q in in vitro assays.
Notes	This Human Angiogenic factor with G patch and FHA domains 1 (AGGF1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGGF1 in samples. An antibody specific for AGGF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGGF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGGF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGGF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55109

Applications	ELISA
Applications Notes	This Human Angiogenic factor with G patch and FHA domains 1 (AGGF1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGGF1 in samples. An antibody specific for AGGF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGGF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGGF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGGF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703178	KTE60962	48 T	$339		Add to Cart
BHE13703178	KTE60962	96 T	$589		Add to Cart
BHE13703178	KTE60962	96 Tx5	$2629		Add to Cart
BHE13703178	KTE60962	96 Tx50	$24569		Add to Cart

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