Human Ang1-7 (Angiotensin 1-7) ELISA Kit

Mfr.No.

E-EL-H5518

Sensitivity

9.38 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Human

Assay Duration

2.5h

Assay Type

Competitive

Description

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang1-7. During the reaction, Ang1-7 in the sample or standard competes with a fixed amount of Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang1-7. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang1-7 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Detection Method

Colormetric

Kit Components

An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells x12 strips 48T: 8 wells x6 strips	-20°C, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL
Concentrated HRP Conjugate (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL	-20°C(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25x)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8°C(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Recovery

80%-120%. The recovery of Ang1-7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-103	97
EDTA plasma(n=8)	94-104	99
Cell culture media(n=8)	88-100	95

Linearity

Samples were spiked with high concentrations of Human Ang1-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	84-97	85-95	95-111
1:2	Average (%)	91	90	103
1:4	Range (%)	85-96	90-106	105
1:4	Average (%)	90	97	105
1:8	Range (%)	87-100	87-102	99-113
1:8	Average (%)	92	94	106
1:16	Range (%)	89-103	89-104	99-110
1:16	Average (%)	96	96	104

Specificity

This kit recognizes Ang1-7 in samples. No significant cross-reactivity or interference between Ang1-7 and analogues was observed.

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Precision

Both intra-CV and inter-CV are < 10%.
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Ang1-7 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Ang1-7 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	46.10	155.10	482.30	46.20	149.20	518.80
Standard deviation	2.50	9.30	23.60	2.40	6.10	22.30
CV (%)	5.42	6.00	4.89	5.19	4.09	4.30

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Human Ang1-7 .During the reaction, Human Ang1-7 in the sample or standard competes with a fixed amount of Human Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang1-7 Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Human Ang1-7 concentrations in serum, plasma and other biological fluids.

UniProt ID	P01019

Applications	ELISA
Applications Notes	This ELISA kit applies to the in vitro quantitative determination of Ang1-7 concentrations in serum, plasma and other biological fluids.
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Storage	2-8°C/-20°C,12 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10302631	E-EL-H5518	96T	$609		Add to Cart
BHE10302631	E-EL-H5518	48T	$487		Add to Cart

Human Ang1-7 (Angiotensin 1-7) ELISA Kit

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