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Home / ELISA Kit

Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit

SKU : BHE13702223

The deduced 208-amino acid AWP1 protein has a calculated molecular mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216. AWP1 has 2 conserved zinc finger domains: ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic reticulum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.

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Mfr.No.	KTE60003
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit employs a two-site sandwich ELISA to quantitate ZFAND6.
Alternative Names	ZFAND6; AWP1; ZA20D3; ZFAND5B; protein associated with PRK1; zinc finger; A20 domain containing 3
Detection Method	Colorimetric
Kit Components	Human AN1-type zinc finger protein 6 microplate Human AN1-type zinc finger protein 6 standard Human AN1-type zinc finger protein 6 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZFAND6. No significant cross-reactivity or interference between Human ZFAND6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced 208-amino acid AWP1 protein has a calculated molecular mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216. AWP1 has 2 conserved zinc finger domains: ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic reticulum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.
Notes	This Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit employs a two-site sandwich ELISA to quantitate ZFAND6 in samples. An antibody specific for ZFAND6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFAND6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFAND6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFAND6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	54469

Applications	ELISA
Applications Notes	This Human AN1-type zinc finger protein 6 (ZFAND6) ELISA Kit employs a two-site sandwich ELISA to quantitate ZFAND6 in samples. An antibody specific for ZFAND6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFAND6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFAND6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFAND6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702223	KTE60003	48 T	$339		Add to Cart
BHE13702223	KTE60003	96 T	$589		Add to Cart
BHE13702223	KTE60003	96 Tx5	$2629		Add to Cart
BHE13702223	KTE60003	96 Tx50	$24569		Add to Cart

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