Matrices listed below were spiked with certain level of AMSH and the recovery rates were calculated by comparing the measured value to the expected amount of AMSH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
97
EDTA plasma(n=5)
89-103
98
heparin plasma(n=5)
86-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AMSH ELISA Kit allows for the in vitro quantitative determination of AMSH concentrations in serum, plasma, tissue homogenates and other biological fluids.